Everything about cytometers

Everything about cytometers

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Data-enriched characterization of mobile populations. Morphological facts from photos can include to your richness of flow cytometry information. For instance, the determine exhibits an usually regular apoptosis assay making use of Annexin V and PI, including mobile imaging to characterize cells in Just about every inhabitants to expose morphologically unique attributes. These insights couldn't have been obtained from multiplex staining by itself.

No matter if you’re a beginner or an authority, investigate our resources to progress your investigate and expertise in flow cytometry.

Incorporate acoustic focusing know-how with significant-parameter Examination for flexible spectral unmixing and traditional workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing with a higher-velocity brightfield digicam for simultaneous superior throughput flow cytometry and significant resolution brightfield imaging.

*Pricing is indicative only and is subject to vary. Please Get hold of your Agilent agent with any queries. The information set forth herein is not really a proposal or simply a commitment, each of which can be subject to credit rating acceptance, terms and conditions, and availability with Agilent. Agilent Monetary Answers is offered in selected international locations.

On top of that, it really is illuminating to determine the number of doublets slip through conventional singlet gates, and good to verify the position of unforeseen double-optimistic functions in my analysis."

Optimizing gating technique. Even robust manual singlet gating is error-vulnerable and stays a subjective choice position in Practically all flow cytometry assays. Imaging can be used to substantiate and alter gates to incorporate only solitary cells of curiosity.

Dependable picture high quality even at significant throughput Acoustic concentrating plus a large-pace digital camera Merge to picture these T cells persistently at very low or superior flow costs. Effortlessly modify focus and digicam settings to fulfill experimental prerequisites.

Early indications of this contamination experienced to begin with been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also permits you to use morphological functions to discover attention-grabbing subpopulations that would not be evident from flow cytometry information by itself.

Consumers can perspective event illustrations or photos in serious-time to substantiate sample high quality, optimize protocols, and file the morphology of every mobile population discovered for Examination. Imaging seize also Positive aspects from acoustic focusing, which helps to placement the cells to ensure that a pointy, centered impression is obtained.

Seem waves empower precise cell alignment, causing Extraordinary info good quality and ~10X more quickly sample flow fees.

We Formerly shown the power of imaging Automobile-T/Ramos mobile interactions. Let’s examine just the population of finest curiosity, the double positive activities, To find out more. We are able to now use extended morphological parameters (here, circularity and skewness of depth) to further more examine the options of these populations and refine gating on these activities, increasing details robustness.

Leverage the power of Highly developed technologies and innovation to speed up your discoveries. Belief while get more info in the Attune Flow Cytometer family members to provide the dependability, velocity, and accuracy that established your function aside.

Versatile payment prepare from Agilent Economical Solutions enable you to get the significant instrumentation you need for instant challenge, or to improve your lab.

Gating on these extended parameters lets you quickly and accurately identify populations of fascination and make sure gating strategy with little or no manual review. The Attune computer software provides use of state-of-the-artwork equipment learning analysis applications inside of an expertly developed consumer interface, making it extremely user friendly.

As the cell line had Earlier been analyzed to the Attune CytPix Flow Cytometer, the scientists went back again to the images and had been in a position to doc the microbial infection no less than five times previously.

Agilent Benefit Promise guarantees you at the very least a decade of instrument use from the date you purchase. Usually, We'll credit score you Together with the residual value of the process towards an upgraded model.

What gave the impression to be a homogeneous population is really extra assorted—an Perception that needs to be regarded when interpreting benefits.